Cell Markers and Genetic Abnormalities for Hodgkin and Non-Hodgkin Lymphomas

HODGKIN LYMPHOMA

Hodgkin lymphoma with a Reed-Sternberg cell

In the center of the photomicrograph is a classic Reed-Sternberg cell,a binucleate cell with large “owl’s eyes” eosinophilic nucleoli.

Hodgkin lymphoma, nodular sclerosis variant

Note the nodules of lymphocytes and other hematopoietic cells divided by broad fibrous septae.

Hodgkin lymphoma, mixed cellularity variant

Note the Reed-Sternberg cell (arrowhead). The other cell types in this image are reactive (only the Reed-Sternberg cell is neoplastic) and include neutrophils, lymphocytes, and, prominently in this image,eosinophils.

NON-HODGKIN LYMPHOMA

Spleen, non-Hodgkin lymphoma. This spleen exhibits uniform multicentric involvement of the white pulp by a malignant lymphoma. These changes contribute to generalized splenomegaly.

Follicular lymphoma

This lymph node exhibits the characteristic low power features of follicular lymphoma, tightly packed lymphoid follicles of approximately equal size.

Follicular lymphoma involves this hilar lymph node. The black material (arrow) represents normal lymphoid parenchyma with anthracotic pigment displaced 

to one side by the expanding tan lymphomatous process.

Diffuse large B-cell lymphoma

The photomicrograph shows a sheet of monotonous large neoplastic cells with prominent nucleoli.

Burkitt lymphoma

This photomicrograph illustrates the characteristic low power features of Burkitt lymphoma, a sheet of neoplastic cells interspersed with punctate clearings (“starry sky pattern”). The punctate clearings are macrophages engulfing cellular debris. Burkitt lymphoma is an aggressive rapidly growingneoplasm with abundant cellular turnover, hence the presence of the macrophages

Hairy cell leukemia

In the upper left corner is a single neoplastic lymphoid cell. Note the fine hair-like projections from its surface.


General Category Specific Name of Genetic Abnormality of Lymphoma Lymphoma Cell Markers (if associated with one)

Hodgkin lymphoma 

• NS-HL CD 15, 30+ EBV
• MC-HL CD 15, 30+ EBV+
• LP-HL CD 20, EMA + CD 15, 30

Non-Hodgkin lymphoma 

• Follicular lymphoma CD 19,20, and 10+ t(14;18)–bcl-2 surface Ig, bcl-2+ 3q27 abnormalities

• Diffuse large B-cell CD 19,20, and 79a+ t(14;18)–bcl-2 lymphoma Surface Ig+ 3q27 abnormalities

• Burkitt lymphoma CD 10, 19 and 20+ t(8;14)–MYC Bcl-6 and surface Ig+

• Precursor T-cell lymphoblastic Tdt, CD 2 and 7+ Abnormalities of TAL1 leukemia/lymphoma

• Mantle cell lymphoma CD 19,20 and 5+ t(11;14)–cyclin D1 Surface Ig+ CD23

• MALToma CD19, 20 t(1;14)–bcl-10 t(11;18)

• Hairy cell leukemia CD 19, 20, 11c and 103 TRAP+

NS-HL, nodular sclerosis Hodgkin lymphoma; EBV, Epstein-Barr virus; EMA, epithelial membrane antigen; MC-HL, mixed cellularity Hodgkin lymphoma; LPHL,
lymphocyte predominant Hodgkin lymphoma; TRAP, tartrate-resistant acid phosphatase.